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Chicken IgY

Immunoglobulin Y

Immunoglobulin Y (abbreviated as IgY) is a type of immunoglobulin which is the major antibody in bird, reptile, and lungfish blood. It is also found in high concentrations in chicken egg yolk. As with the other immunoglobulins, IgY is a class of proteins which are formed by the immune system in reaction to certain foreign substances, and specifically recognize them.

IgY is often mislabelled as Immunoglobulin G (IgG) in older literature, and sometimes even in commercial product catalogues, due to its functional similarity to mammalian IgG and Immnunoglobulin E (IgE). However, this older nomenclature is obsolete, since IgY differs both structurally and functionally from mammalian IgG, and does not cross-react with antibodies raised against mammalian IgG.

Since chickens can lay eggs almost every day, and the yolk of an immunized hen’s egg contains a high concentration of IgY, chickens are gradually becoming popular as a source of customized antibodies for research. (Usually, mammals such as rabbits or goats are injected with the antigen of interest by the researcher or a contract laboratory.)

Chicken and avian IgY is a fast growing field of research due to several of the unique and advantageous properties of the IgY immunoglobulin. It’s widely known that IgY does not activate mammalian complement factors, making it extremely useful in the development of assays for mammalian serums. IgY also has the beneficial property of being present not only in avian serum but also being the sole immunoglobulin in egg yolk. This allows for the potential for cleaner preps of IgY due to the lack of other immunoglobulins in egg yolk. One last crucial detail about IgY, is that neither Protein A or Protein G are capable of binding it, making purification difficult at large scale due to the lack of affinity media. LigaTrap® Technologies continues to make groundbreaking strides in the field of immunoglobulin purification by releasing our LigaTrap® Chicken IgY Purification resin. Capable of binding IgY at ≥15mg/ml from both serum and yolk samples.

Part # Name Amount Price
LT-142KIT

LigaTrap Chicken IgY Kit

1 Kit $349.00 Add to Cart
LT-142

LigaTrap Chicken IgY Resin

5 ml $555.00 Add to Cart
LT-142-1x1ml

LigaTrap® Chicken IgY Purification Column

1 ml $125.00 Add to Cart
LT-142-1x5ml

LigaTrap® Chicken IgY Purification Column

1 x 5 ml $595.00 Add to Cart
LT-142-3x1ml

LigaTrap® Chicken IgY Purification Column

3 x 1 ml $365.00 Add to Cart

Chicken IgY Yield and Purity

LigaTrap® Chicken IgY Purification resin capable of high yields and >95% purity, compared to Protein A and Protein G with yields below detection limit.

Chicken yolk was loaded over LigaTrap® Chicken IgY Purification resin and GE HP Resin with a five-minute residence time. Yields in histogram were determined using absorbance measurements at 280 nm. All manufacturer recommended chromatographic protocols were followed.

COMPARABILITY
SDS PAGE


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