Goat IgG Purification Resin and Columns
Immunoglobulin G (IgG) Represents approximately 75% of serum antibodies as the most common type of antibody found in blood circulation. IgG molecules are created and released by plasma B cells. Each IgG has two antigen binding sites. Human IgG1 and IgG3 are highly active in initiating Antibody Dependent Cell-Mediated Cytotoxicity (ADCC) and Complement Dependent Cytotoxicity (CDC). In contrast, IgG2 and IgG4 are much less capable in evoking such an immune response. As a result, IgG1 and IgG3 are the most suitable for therapeutic use against cancer cells. LigaTrap also offers the advantages of a milder (more basic) pH than other widely used resins, has little or no leaching, and resin can be reused up to 100 cycles.
| Part # | Name | Amount | Price | |
| LT-136KIT | Goat IgG Mircospin Column Kit w/ Buffers |
KIT-10x0.1mL | $308.70 | Add to Cart |
| LT-136-1ml KIT | Goat IgG 1mL Prepacked Column Kit w/ Buffers |
KIT-1x1mL | $236.25 | Add to Cart |
| LT-136-5ml KIT | Goat IgG 5mL Prepacked Column Kit w/ Buffers |
KIT-1x5mL | $840.00 | Add to Cart |
| LT-136 | Goat IgG Loose Resin-5mL |
5mL | $472.50 | Add to Cart |
| LT-136-10ml | Goat IgG Loose Resin-10mL |
10mL | $750.00 | Add to Cart |
| LT-136-25ml | Goat IgG Loose Resin-25mL |
25mL | $959.20 | Add to Cart |
| LT-136-50ml | Goat IgG Loose Resin-50mL |
50mL | $1,398.20 | Add to Cart |
| LT-136-100ml | Goat IgG Loose Resin-100mL |
100mL | $1,756.00 | Add to Cart |
| LT-136-MSC | Goat IgG Microspin Columns without buffers |
10x0.1mL | $131.25 | Add to Cart |
| LT-136-1x1ml | Goat IgG Prepacked Purification Column-1mL |
1x1mL | $105.00 | Add to Cart |
| LT-136-1x5ml | Goat IgG Prepacked Purification Column-5mL |
1x5mL | $498.75 | Add to Cart |
| LT-136-3x1ml | Goat IgG Prepacked Purification Columns-3x1mL |
3x1mL | $309.75 | Add to Cart |
Goat IgG Yield and Purity
Goat IgG was purified from goat serum for loading over LigaTrap Purification Resin. It was compared to goat serum loaded over Pierce Protein A Plus, and Thermo Protein G. The LigaTrap purified resin demonstrated a static binding capacity comparable with both Protein A and Protein G, with purity >90%. All manufacturer recommended chromatographic protocols were followed. In addition to increased yields and purity levels, LigaTrap technologies addresses many of the shortfalls related to standard affinity ligands including challenges of elution pH, leaching, proteolysis and repeated sanitization associated with other common purification methodologies.
