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Guard Column

Currently, chromatography production column resins’ binding capabilities are never fully used, hold times are long, and equipment only runs a small percentage of the time.  Many solutions are being investigated to improve the bioseparations platform including continuous multicolumn processes.  The LigaTrap guard column is a perfect solution using this approach for monoclonal antibodies.

  • Unlike the common filtering of contaminants by size exclusion technology, the Guard Column is based on affinity chromatography and the ligand capable of being placed on size exclusion filters.
  • Guard column resin uses very stable peptide and peptoid ligands and in cleaning protocols LigaTrap recommends harsher base cleaning than industry standard.  Long cycle life is well preserved despite more thorough cleaning.  These attributes result in low cost prepossessing step per cycle of use.
  • The column captures wider ranges of impurities and undesired molecules so they never reach expensive production columns.  Being more robust than Protein A or G for purification, this preprocessing lowers costs of the production column because of fewer impurities and lower volume.
  • The column is adaptable to continuous bioprocessing by placing 2 or 3 guard columns in the production line as it is adaptable for use of 1 active Guard Column in preprocessing while the other(s) undergo cleaning and readiness to be put into production.
  • Guard column residence time requirements are relatively short at 1 to 5 minutes.

In one study, “not only did the researchers find that the platform would reduce equipment costs by more than 55%, footprint by 32%, water use by 85%, buffer requirements by 58%, and overall time by 45%, but that it reduced overall unit operation costs by 21% at the 3-kg level, and by 29% at the 15-kg level, suggesting continuous downstream processes’ potential.”

Guard Process Column Use

The LigaTrap Guard Column is the solution.

Part # Name Amount Price
LT-136-1x1ml LigaTrap® Goat IgG Purification Column 1 ml $100.00 Add to Cart
LT-136-1x5ml LigaTrap® Goat IgG Purification Column 1 x 5 ml $475.00 Add to Cart
LT-136-3x1ml LigaTrap® Goat IgG Purification Column 3 x 1 ml $295.00 Add to Cart
LT-095-1x1ml LigaTrap® Human IgG Purification Column 1 ml $100.00 Add to Cart
LT-095-1x5ml LigaTrap® Human IgG Purification Column 1 x 5 ml $475.00 Add to Cart
LT-095-3x1ml LigaTrap® Human IgG Purification Column 3 x 1 ml $295.00 Add to Cart
LT-137-1x1ml LigaTrap® Mouse IgG Purification Column 1 ml $100.00 Add to Cart
LT-137-1x5ml LigaTrap® Mouse IgG Purification Column 1 x 5 ml $475.00 Add to Cart
LT-137-3x1ml LigaTrap® Mouse IgG Purification Column 3 x 1 ml $295.00 Add to Cart
LT-139-1x1ml LigaTrap® Rabbit IgG Purification Column 1 ml $100.00 Add to Cart
LT-139-1x5ml LigaTrap® Rabbit IgG Purification Column 1 x 5 ml $475.00 Add to Cart
LT-139-3x1ml LigaTrap® Rabbit IgG Purification Column 3 x 1 ml $295.00 Add to Cart
LT-138-1x1ml LigaTrap® Rat IgG Purification Column 1 ml $100.00 Add to Cart
LT-138-1x5ml LigaTrap® Rat IgG Purification Column 1 x 5 ml $475.00 Add to Cart
LT-138-3x1ml LigaTrap® Rat IgG Purification Column 3 x 1 ml $295.00 Add to Cart
LT-141-1x1ml LigaTrap® Sheep IgG Purification Column 1 ml $100.00 Add to Cart
LT-141-1x5ml LigaTrap® Sheep IgG Purification Column 1 x 5 ml $475.00 Add to Cart
LT-141-3x1ml LigaTrap® Sheep IgG Purification Column 3 x 1 ml $295.00 Add to Cart
LT-144-1x1ml LigaTrap® Llama IgG Purification Column 1 ml $100.00 Add to Cart
LT-144-1x5ml LigaTrap® Llama IgG Purification Column 1 x 5 ml $475.00 Add to Cart
LT-144-3x1ml LigaTrap® Llama IgG Purification Columns 3 x 1 ml $295.00 Add to Cart



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