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Llama IgG Resin and Columns

Llama antibodies are coming into greater use due to their unique characteristics including small size.  Their steric hindrance is reduced, allowing them to reach hardly accessible antigens that conventional antibodies cannot, such as active sites of enzymes (useful to inhibit enzyme activity) as well as smaller enzymes, such as lysozymes or ribonucleases.  Additionally, Llama antibodies have the capacity to penetrate tissues compared to conventional antibodies, allowing a more precise staining of tissue sections when performing immunohistochemistry.  The high refolding capacity of the VHH region allow them to be more thermodynamically stable and resistant to high temperatures (up to 90°C / 194°F) and extreme pH conditions, such as gastric acid (pH 14).

LigaTrap technology separates itself by having the highest quality chromatographic purification for IgG on the market today. The advantages LigaTrap IgG Resin include capture IgG subtypes, no immunogenicity or leaching, mild elution conditions, and can be reused after sanitization up to 100 times. LigaTrap IgG Purification Resin is capable of processing and purifying monoclonal antibodies form cell culture supernatant, ascites fluid, hybridoma, and other sources of recombinant IgG.

Product Descriptions

Purification kits are offered in microspin column, 1mL prepacked column, and 5mL prepacked column formats. Each kit includes the appropriate buffers and protocol for performing purifications.

Prepacked columns are offered in 1mL and 5mL formats for our various lines of antibody affinity chromatography resins. Prepacked columns come in kit formats or as standalone products.

Loose resin is available in various volume sizes which can be packed for end user’s specific purification needs. For bulk resin orders larger than 100mL, please inquire.

This product is not recommended in serum purification applications due to cross-reactivity with other immunoglobulins. Please consult LigaTrap for protocols that may improve results in serum.

Part # Name Amount Price
LT-144KIT

Llama IgG Mircospin Column Kit w/ Buffers

KIT-10x0.1 mL $318.00 Add to Cart
LT-144-1ml KIT

Llama IgG 1mL Prepacked Column Kit w/ Buffers

KIT-1x1 mL $244.00 Add to Cart
LT-144-5ml KIT

Llama IgG 5mL Prepacked Column Kit w/ Buffers

KIT-1x5 mL $866.00 Add to Cart
LT-144

Llama IgG Loose Resin-5mL

5 mL $487.00 Add to Cart
LT-144-10ml

Llama IgG Loose Resin-10mL

10 mL $773.00 Add to Cart
LT-144-25ml

Llama IgG Loose Resin-25mL

25 mL $1,136.20 Add to Cart
LT-144-50ml

Llama IgG Loose Resin-50mL

50 mL $1,657.15 Add to Cart
LT-144-100ml

Llama IgG Loose Resin-100mL

100 mL $2,080.35 Add to Cart
LT-144-MSC

Llama IgG Microspin Columns without buffers

10x0.1 mL $136.00 Add to Cart
LT-144-1x1ml

Llama IgG Prepacked Purification Column-1mL

1x1 mL $109.00 Add to Cart
LT-144-1x5ml

Llama IgG Prepacked Purification Column-5mL

1x5 mL $514.00 Add to Cart
LT-144-3x1ml

Llama IgG Prepacked Purification Columns-3x1mL

3x1 mL $320.00 Add to Cart

Llama IgG Yield and Purity

LigaTrap Llama IgG Purification resin stands above the competition (Protein A and Protein G) when you look at yield, recovery of all three isotypes, and aggregate reduction. All of this is accomplished using a milder pH (4.0-5.0) than Protein A and G which use very low pH elutions of 2.5-2.8. With >6X recovery compared to Protein A, use for up to 100 cycles, and the ability to bind all three Llama IgG subtypes as opposed to Protein G, makes LigaTrap Llama IgG Purification resin is a favorite amongst researchers and companies working in the Llama and Camelid IgG field.

COMPARABILITY
SDS PAGE


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