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Llama IgG Resin and Columns

Like all mammals, camelids produce conventional antibodies made of 2 heavy chains and 2 light chains bound together with disulphide bonds in a Y shape (IgG1). However, they also produce two unique subclasses of immunoglobulin G, IgG2 and IgG3, also known as heavy chain IgG (hcIgG). These antibodies are made of only two heavy chains 1, that lack the CH1 region but still bear an antigen binding domain at their N-terminus called VHH.

Advantages for utilizing Llama Antibodies

  • Very interesting characteristics result from their smaller size.
  • their steric hindrance is reduced, allowing them to reach hardly accessible antigens that conventional antibodies can not, such as
  • active sites of enzymes (useful to inhibit enzyme activity) as well as smaller enzymes, such as lysozymes or ribonucleases.
  • their capacity to penetrate tissues is increased when compared to conventional antibodies, allowing a more precise staining of
  • tissue sections when performing immunohistochemistry.

Furthermore, the high refolding capacity of the VHH region allow them to be thermodynamically more stable and especially to resist to high temperatures (up to 90°C / 194°F) 10, 11, 12, 13 and extreme pH, such as gastric acid 14.

Part # Name Amount Price
LT-144KIT

LigaTrap Llama IgG Kit

1 Kit $294.00 Add to Cart
LT-144

LigaTrap Llama IgG Resin

5 ml $450.00 Add to Cart
LT-144-1x1ml

LigaTrap® Llama IgG Purification Column

1 ml $100.00 Add to Cart
LT-144-1x5ml

LigaTrap® Llama IgG Purification Column

1 x 5 ml $475.00 Add to Cart
LT-144-3x1ml

LigaTrap® Llama IgG Purification Columns

3 x 1 ml $295.00 Add to Cart

Llama IgG Yield and Purity

LigaTrap® Llama IgG Purification resin stands above the competition (Protein A and Protein G) when you look at yield, recovery of all three isotypes, and aggregate reduction. All of this is accomplished using a milder pH (4.0-5.0) than Protein A and G which use very low pH elutions of 2.5-2.8. With >6X recovery compared to Protein A and the ability to bind all three Llama IgG subtypes as opposed to Protein G, makes LigaTrap® Llama IgG Purification resin is a favorite amongst researchers and companies working in the Llama and Camelid IgG field.

COMPARABILITY
SDS PAGE


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