Rabbit IgG Purification Resin and Columns
Immunoglobulin G (IgG) Represents approximately 75% of serum antibodies in humans, IgG is the most common type of antibody found in blood circulation. IgG molecules are created and released by plasma B cells. Each IgG has two antigen binding sites. Human IgG1 and IgG3 are highly active in initiating Antibody Dependent Cell-Mediated Cytotoxicity (ADCC) and Complement Dependent Cytotoxicity (CDC). In contrast, IgG2 and IgG4 are much less capable in evoking such an immune response. As a result, IgG1 and IgG3 are the most suitable for therapeutic use against cancer cells.
Normal Rabbit IgG is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions.
Rabbit monoclonal antibody. Efforts have been ongoing for the last few decades to generate rabbit monoclonal antibodies. An antibody generated in rabbits is especially important to those antigens that are not immunogenic in rodents, which are the most commonly used host animals for monoclonal antibody production.
| Part # | Name | Amount | Price | |
| LT-139KIT | LigaTrap Rabbit IgG Kit |
1 Kit | $294.00 | Add to Cart |
| LT-139 | LigaTrap Rabbit IgG Resin |
5 ml | $450.00 | Add to Cart |
| LT-139-1x1ml | LigaTrap® Rabbit IgG Purification Column |
1 ml | $100.00 | Add to Cart |
| LT-139-1x5ml | LigaTrap® Rabbit IgG Purification Column |
1 x 5 ml | $475.00 | Add to Cart |
| LT-139-3x1ml | LigaTrap® Rabbit IgG Purification Column |
3 x 1 ml | $295.00 | Add to Cart |
Rabbit Serum Yield and Purity
Rabbit serum was purified from 1.44 ml of 11.1 mg/ml, 160 microliters of water and 400 microliters of sample diluent creating 2 ml at 8 mg/ml of Rabbit serum loading over LigaTrap® Rabbit Purification resin. It was compared to .87 ml of 22.9 mg/ml of rabbit serum with 750 microliters of sample diluent and 730 microliters of water creating 2 ml at 8 mg/ml of rabbit serum. The samples were loaded over Pierce Protein A Plus, and Pierce Protein G. Yields were determined. All manufacturer recommended chromatographic protocols were followed.
