Custom Protein Purification
LigaTrap® Technologies has many years of combined expertise in purifying a wide variety of proteins including monoclonal and polyclonal antibodies, recombinant proteins, fusion proteins, affinity-tagged proteins, and native proteins. We offer purification from many formats including (but not limited to) cell culture supernatant, ascites fluid, serum/antiserum, bacterial and mammalian expression systems, avian eggs, etc. We utilize AKTA systems to automate most purification cycles, allowing for increased efficiencies, lower costs, and shorter lead times for our customers. Typical chromatographic strategies used by our purification facility are listed below:
- LigaTrap® Resin Purification
- IgG’s, IgY’s, IgA’s, IgM’s
- Human, Mouse, Rat and many other species
- Protein A/G/L Purification
- Affinity Purification
- Antigen Immunoaffinity
- Conventional Chromatography:
Analytical testing is an essential part of the proper evaluation and assurance that purified biologicals meet product specifications. Additionally, to ensure integrity of data, it is necessary to have a third-party independent analysis done to confirm internal analytical results. LigaTrap® Technologies offers a variety of different testing services for analysis of purified proteins. Some of these services include SDS-PAGE (native and denaturing), HPLC, Western Blot, ELISA, A280 determination, endotoxin, and bioburden. Discounted pricing is available for customers that have multiple samples and/or multiple assays to be conducted.
Our expert Team of scientists can develop a downstream process that provides efficiency and maximized recovery. Our methods include standard chromatography and our own proprietary technologies. The LigaTrap team works as an extension of your company to review, develop and transfer processes for both antisera, ascites and cell culture.
Optimization using LigaTrap® Purification Resin
It is the goal of LigaTrap® Technologies to improve the efficiency of antibody purifications by using our novel LigaTrap® affinity resins over traditional chromatographic methodologies. In an effort to convince customers that there is an alternative, and in many instances, a better solution to their conventional platform antibody purification technology, we have introduced a new initiative to offer discounted purification services as they relate to and utilize one of our LigaTrap® purification resins in the process. We employ high throughput methodology to develop and optimize a custom purification process using a resin from our LigaTrap® product portfolio that is specific to the customer’s species and immunoglobulin subclass as well as their particular application. Finally, this customized process can then be transferred to the customer so that they can then implement the optimized process and scale up purification in their own lab.