Human IgA Purification Resin and Columns
Immunoglobulin A (IgA) monoclonal antibodies have become increasingly favorable for their use as therapeutic tools, while remaining popular for their research and diagnostic applications. More IgA based therapeutics are being developed now than ever before due to a better understanding of the roles it plays in the immune system. In the past, IgA has been one of the more difficult antibodies to purify due to the lack of a robust and high capacity affinity resin.
With binding capacities of ≥15mg IgA/mL resin, LigaTrap Human IgA purification resin far exceeds the capacity of PierceTM Jacalin and other lectin-based resins commonly used. Additionally, LigaTrap Human IgA Resin does not require the use of sugar (galactose, sucrose, etc.) based elution buffers, therefore simplifying further downstream processing. The resin binds both IgA1 and IgA2 subclasses.
LigaTrap technology separates itself by having the highest quality chromatographic purification for IgA on the market today. The advantages LigaTrap IgA Resin include capture both heavy and light chain subtypes of IgA, no immunogenicity or leaching, mild elution conditions, and can be reused after sanitization up to 100 times. LigaTrap Human IgA Purification Resin is capable of processing and purifying monoclonal antibodies form cell culture supernatant, ascites fluid, hybridoma, and other sources of recombinant IgA.
Purification kits are offered in microspin column, 1mL prepacked column, and 5mL prepacked column formats. Each kit includes the appropriate buffers and protocol for performing purifications.
Prepacked columns are offered in 1mL and 5mL formats for our various lines of antibody affinity chromatography resins. Prepacked columns come in kit formats or as standalone products.
Loose resin is available in various volume sizes which can be packed for end user’s specific purification needs. For bulk resin orders larger than 100mL, please inquire.
This product is not recommended in serum purification applications due to cross-reactivity with other immunoglobulins. Please consult LigaTrap for protocols that may improve results in serum.
Human IgA Mircospin Column Kit w/ Buffers
|KIT-10x0.1 mL||$341.00||Add to Cart|
Human IgA 1mL Prepacked Column Kit w/ Buffers
|KIT-1x1 mL||$269.40||Add to Cart|
Human IgA 5mL Prepacked Column Kit w/ Buffers
|KIT-1x5 mL||$962.00||Add to Cart|
Human IgA Lose Resin-5 mL
|5 mL||$522.00||Add to Cart|
Human IgA Loose Resin-10mL
|10 mL||$828.00||Add to Cart|
Human IgA Loose Resin-25mL
|25 mL||$1,058.00||Add to Cart|
Human IgA Loose Resin-50mL
|50 mL||$1,542.00||Add to Cart|
Human IgA Loose Resin-100mL
|100 mL||$1,936.00||Add to Cart|
Human IgA Microspin Columns without buffers
|10x0.1 mL||$146.00||Add to Cart|
Human IgA Prepacked Purification Column-1mL
|1x1 mL||$124.40||Add to Cart|
Human IgA Prepacked Purification Column-5mL
|1x5 mL||$562.00||Add to Cart|
Human IgA Prepacked Purification Columns-3x1mL
|3x1 mL||$351.20||Add to Cart|
IgA Yield and Purity
Purified Human IgA1 was spiked into PBS buffer and then loaded over LigaTrap Human IgA Purification resin and Pierce Jacalin agarose at a load of 20mg IgA/ml resin. A residence time of five minutes was used and all manufacturer recommended protocols were followed.