IgG Purification Resin and Columns
Immunoglobulin G (IgG) Represents approximately 75% of serum antibodies in humans, IgG is the most common type of antibody found in blood circulation. IgG molecules are created and released by plasma B cells. Each IgG has two antigen binding sites. Human IgG1 and IgG3 are highly active in initiating Antibody Dependent Cell-Mediated Cytotoxicity (ADCC) and Complement Dependent Cytotoxicity (CDC). In contrast, IgG2 and IgG4 are much less capable in evoking such an immune response. As a result, IgG1 and IgG3 are the most suitable for therapeutic use against cancer cells.
| Part # | Name | Amount | Price | |
| LT-095KIT | LigaTrap Human IgG Kit |
1 Kit | $294.00 | Add to Cart |
| LT-095 | LigaTrap Human IgG Resin |
5 ml | $450.00 | Add to Cart |
| LT-095-1x1ml | LigaTrap® Human IgG Purification Column |
1 ml | $100.00 | Add to Cart |
| LT-095-1x5ml | LigaTrap® Human IgG Purification Column |
1 x 5 ml | $475.00 | Add to Cart |
| LT-095-3x1ml | LigaTrap® Human IgG Purification Column |
3 x 1 ml | $295.00 | Add to Cart |
IgG Yield and Purity
Human IgG was purified from CHO spiked media containing PBS and an 80 mg/ml load by passing the sample overLigaTrap® Human IgG resin, Pierce Plus Protein A and Pierce Protein G. TheLigaTrap® purified resin demonstrated a static binding capacity higher than Protein A and purity >95%. Human IgG subclasses in spiked CHO media with 1% FBS compared to leading competitor Protein A and G demonstrated superior ability ofLigaTrap® Human IgG to purify IgG1 and IgG2. Three different manufacturer recommended binding conditions were used for the leading competitor of IgM purification resin. All manufacturer recommended chromatographic protocols were followed.
