IgG Purification Resin and Columns

Immunoglobulin G (IgG) Represents approximately 75% of serum antibodies in humans, IgG is the most common type of antibody found in blood circulation. IgG molecules are created and released by plasma B cells. Each IgG has two antigen binding sites. Human IgG1 and IgG3 are highly active in initiating Antibody Dependent Cell-Mediated Cytotoxicity (ADCC) and Complement Dependent Cytotoxicity (CDC). In contrast, IgG2 and IgG4 are much less capable in evoking such an immune response. As a result, IgG1 and IgG3 are the most suitable for therapeutic use against cancer cells.

Part #	Name	Amount	Price
LT-095KIT	LigaTrap Human IgG Kit	1 Kit	$308.70	Add to Cart



LT-095-1ml KIT	LigaTrap Human IgG 1ml Column Kit	1ml KIT	$236.25	Add to Cart



LT-095-5ml KIT	LigaTrap Human IgG 5ml Column Kit	5ml KIT	$840.00	Add to Cart



LT-095	LigaTrap Human IgG Resin	5 ml	$450.00	Add to Cart



LT-095-10ml	LigaTrap Human IgG Resin	10ml	$750.00	Add to Cart



LT-095-25ml	LigaTrap Human IgG Resin	25ml	$959.20	Add to Cart



LT-095-50ml	LigaTrap Human IgG Resin	50ml	$1,398.20	Add to Cart



LT-095-100ml	LigaTrap Human IgG Resin	100ml	$1,756.00	Add to Cart



LT-095-MSC	LigaTrap Human IgG MicroSpin Columns- Qty 10	MSC	$131.25	Add to Cart



LT-095-1x1ml	LigaTrap^® Human IgG Purification Column	1 ml	$105.00	Add to Cart



LT-095-1x5ml	LigaTrap^® Human IgG Purification Column	1 x 5 ml	$498.75	Add to Cart



LT-095-3x1ml	LigaTrap^® Human IgG Purification Column	3 x 1 ml	$309.75	Add to Cart

	LigaTrap Human IgG Loose Resin Protocol
	LigaTrap Human IgG Prepacked Column Instructions
	LigaTrap Human IgG Purification Kit Instructions

IgG Yield and Purity

Human IgG was purified from CHO spiked media containing PBS and an 80 mg/ml load by passing the sample overLigaTrap® Human IgG resin, Pierce Plus Protein A and Pierce Protein G. TheLigaTrap® purified resin demonstrated a static binding capacity higher than Protein A and purity >95%. Human IgG subclasses in spiked CHO media with 1% FBS compared to leading competitor Protein A and G demonstrated superior ability ofLigaTrap® Human IgG to purify IgG1 and IgG2. Three different manufacturer recommended binding conditions were used for the leading competitor of IgM purification resin. All manufacturer recommended chromatographic protocols were followed.

COMPARABILITY

SDS PAGE

About LigaTrap

LigaTrap® purification kits and resins are convenient and easy-to-use. Simply add the sample to the resin, mix and centrifuge. Not only are LigaTrap® purification protocols simple to follow, but also allow for fast and easy recovery of purified antibodies for screening in your end-use assay. Multiple samples can be processed at the same time, resulting in rapid screening for antibody production. LigaTrap® spin columns only require a pipette and centrifuge, no specialized equipment needed. Each spin column is capable of up to 10 purifications, and each kit has the ability to perform over 100 purifications, making LigaTrap® a cost-effective method for antibody purification.