Human IgG Purification Resin and Columns
Immunoglobulin G (IgG) represents approximately 75% of serum antibodies in humans and is the most common type of antibody found in blood circulation. IgG molecules are created and released by plasma B cells with each IgG molecule possessing two antigen binding sites. The four subclasses of Human IgG are IgG1, IgG2, IgG3, and IgG4 – each of which plays its own role in eliciting immune system responses and is used for a variety of therapeutic research. LigaTrap Human IgG Purification products are capable of purifying all 4 subclasses.
In addition to increased yields and purity levels, LigaTrap Technologies address many of the shortfalls related to standard affinity ligands including challenges. Our peptide ligands provide for a robust lifecycle, mild elution conditions and no immunogenicity or leaching.
| Part # | Name | Amount | Price | |
| LT-095KIT | Human IgG Mircospin Column Kit w/ Buffers |
KIT-10x0.1mL | $318.00 | Add to Cart |
| LT-095-1ml KIT | Human IgG 1mL Prepacked Column Kit w/ Buffers |
KIT-1x1mL | $244.00 | Add to Cart |
| LT-095-5ml KIT | Human IgG 5mL Prepacked Column Kit w/ Buffers |
KIT-1x5mL | $866.00 | Add to Cart |
| LT-095 | Human IgG Loose Resin-5mL |
5mL | $487.00 | Add to Cart |
| LT-095-10ml | Human IgG Loose Resin-10mL |
10mL | $773.00 | Add to Cart |
| LT-095-25ml | Human IgG Loose Resin-25mL |
25mL | $988.00 | Add to Cart |
| LT-095-50ml | Human IgG Loose Resin-50mL |
50mL | $1,441.00 | Add to Cart |
| LT-095-100ml | Human IgG Loose Resin-100mL |
100mL | $1,809.00 | Add to Cart |
| LT-095-MSC | Human IgG Microspin Columns without buffers |
10x0.1mL | $136.00 | Add to Cart |
| LT-095-1x1ml | Human IgG Prepacked Purification Column-1mL |
1x1mL | $109.00 | Add to Cart |
| LT-095-1x5ml | Human IgG Prepacked Purification Column-5mL |
1x5mL | $514.00 | Add to Cart |
| LT-095-3x1ml | Human IgG Prepacked Purification Columns-3x1mL |
3x1mL | $320.00 | Add to Cart |
Human IgG Yield and Purity
Human IgG was purified from CHO spiked media containing PBS and an 80 mg/ml load by passing the sample over LigaTrap Human IgG resin, Pierce Plus Protein A and Pierce Protein G. The LigaTrap purified resin demonstrated a static binding capacity higher than Protein A and purity >95%. Human IgG subclasses in spiked CHO media with 1% FBS compared to leading competitor Protein A and G demonstrated superior ability of LigaTrap Human IgG to purify IgG1 and IgG2. Three different manufacturer recommended binding conditions were used for the leading competitor of IgM purification resin. All manufacturer recommended chromatographic protocols were followed.
