Human IgG Purification Resin and Columns
Immunoglobulin G (IgG) Represents approximately 75% of serum antibodies as the most common type of antibody found in blood circulation. It is the most commonly researched and used in therapeutics of the various antibodies.
LigaTrap technology separates itself by having the highest quality chromatographic purification for IgG on the market today. The advantages LigaTrap IgG Resin include capture IgG subtypes, no immunogenicity or leaching, mild elution conditions, and can be reused after sanitization up to 100 times. LigaTrap IgG Purification Resin is capable of processing and purifying monoclonal antibodies form cell culture supernatant, ascites fluid, hybridoma, and other sources of recombinant IgG.
Product Descriptions
Purification kits are offered in microspin column, 1mL prepacked column, and 5mL prepacked column formats. Each kit includes the appropriate buffers and protocol for performing purifications.
Prepacked columns are offered in 1mL and 5mL formats for our various lines of antibody affinity chromatography resins. Prepacked columns come in kit formats or as standalone products.
Loose resin is available in various volume sizes which can be packed for end user’s specific purification needs. For bulk resin orders larger than 100mL, please inquire.
This product is not recommended in serum purification applications due to cross-reactivity with other immunoglobulins. Please consult LigaTrap for protocols that may improve results in serum.
| Part # | Name | Amount | Price | |
| LT-095KIT | Human IgG Mircospin Column Kit w/ Buffers |
KIT-10x0.1 mL | $318.00 | Add to Cart |
| LT-095-1ml KIT | Human IgG 1mL Prepacked Column Kit w/ Buffers |
KIT-1x1 mL | $244.00 | Add to Cart |
| LT-095-5ml KIT | Human IgG 5mL Prepacked Column Kit w/ Buffers |
KIT-1x5 mL | $866.00 | Add to Cart |
| LT-095 | Human IgG Loose Resin-5mL |
5 mL | $487.00 | Add to Cart |
| LT-095-10ml | Human IgG Loose Resin-10mL |
10 mL | $773.00 | Add to Cart |
| LT-095-25ml | Human IgG Loose Resin-25mL |
25 mL | $1,136.20 | Add to Cart |
| LT-095-50ml | Human IgG Loose Resin-50mL |
50 mL | $1,657.15 | Add to Cart |
| LT-095-100ml | Human IgG Loose Resin-100mL |
100 mL | $2,080.35 | Add to Cart |
| LT-095-MSC | Human IgG Microspin Columns without buffers |
10x0.1 mL | $136.00 | Add to Cart |
| LT-095-1x1ml | Human IgG Prepacked Purification Column-1mL |
1x1 mL | $109.00 | Add to Cart |
| LT-095-1x5ml | Human IgG Prepacked Purification Column-5mL |
1x5 mL | $514.00 | Add to Cart |
| LT-095-3x1ml | Human IgG Prepacked Purification Columns-3x1mL |
3x1 mL | $320.00 | Add to Cart |
Human IgG Yield and Purity
Human IgG was purified from CHO spiked media containing PBS and an 80 mg/ml load by passing the sample over LigaTrap Human IgG resin, Pierce Plus Protein A and Pierce Protein G. The LigaTrap purified resin demonstrated a static binding capacity higher than Protein A and purity >95%. Human IgG subclasses in spiked CHO media with 1% FBS compared to leading competitor Protein A and G demonstrated superior ability of LigaTrap Human IgG to purify IgG1 and IgG2. Three different manufacturer recommended binding conditions were used for the leading competitor of IgM purification resin. All manufacturer recommended chromatographic protocols were followed.
