IgG Purification Resin and Columns
LigaTrap® Technologies is excited to offer our popular Mouse IgG purification resin. LigaTrap® Mouse IgG purification line can bind all mouse IgG isotypes (IgG1, IgG2a, IgG2b, and IgG3) from polyclonal and monoclonal sources at a capacity of ≥20mg Mouse IgG/ml of resin. It is important to note that Protein A and Protein G have weaker affinity for mouse IgG1 compared to all other mouse IgG subclasses. We offer LigaTrap® Rat IgG Purification resin in all of our standard formats including bulk resin (LT-138), purification kit (LT-138KIT), 1mL (LT-138-1x1mL) and 5mL (LT-138-1x5mL) prepacked columns.
| Part # | Name | Amount | Price | |
| LT-137KIT | LigaTrap Mouse IgG Kit |
1 Kit | $294.00 | Add to Cart |
| LT-137 | LigaTrap Mouse IgG Resin |
5 ml | $450.00 | Add to Cart |
| LT-137-1x1ml | LigaTrap® Mouse IgG Purification Column |
1 ml | $100.00 | Add to Cart |
| LT-137-1x5ml | LigaTrap® Mouse IgG Purification Column |
1 x 5 ml | $475.00 | Add to Cart |
| LT-137-3x1ml | LigaTrap® Mouse IgG Purification Column |
3 x 1 ml | $295.00 | Add to Cart |
IgG Yield and Purity
Purified polyclonal mouse IgG was spiked into a cell culture supernatant containing 5% fetal bovine serum and loaded over LigaTrap® Rat IgG Purification resin, Pierce™ Protein A and Protein G Plus at a load of 8mg IgG/ml resin with a five-minute residence time. Yields in histogram were determined using absorbance measurements at 280 nm. All manufacturer recommended chromatographic protocols were followed.
Mouse ascites fluid expressing monoclonal mouse IgG1-anti-IL6 (Interleukin 6) was loaded over LigaTrap® mouse IgG Purification resin, Pierce™ Protein A and Protein G Plus with a five-minute residence time. Yields in histogram were determined using ELISA. All manufacturer recommended chromatographic protocols were followed.
