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IgG Purification Resin and Columns

LigaTrap® Technologies is excited to offer our popular Mouse IgG purification resin. LigaTrap® Mouse IgG purification line can bind all mouse IgG isotypes (IgG1, IgG2a, IgG2b, and IgG3) from polyclonal and monoclonal sources at a capacity of ≥20mg Mouse IgG/ml of resin. It is important to note that Protein A and Protein G have weaker affinity for mouse IgG1 compared to all other mouse IgG subclasses. We offer LigaTrap® Rat IgG Purification resin in all of our standard formats including bulk resin (LT-138), purification kit (LT-138KIT), 1mL (LT-138-1x1mL) and 5mL (LT-138-1x5mL) prepacked columns.

Part # Name Amount Price
LT-137KIT

LigaTrap Mouse IgG Kit

1 Kit $294.00 Add to Cart
LT-137

LigaTrap Mouse IgG Resin

5 ml $450.00 Add to Cart
LT-137-1x1ml

LigaTrap® Mouse IgG Purification Column

1 ml $100.00 Add to Cart
LT-137-1x5ml

LigaTrap® Mouse IgG Purification Column

1 x 5 ml $475.00 Add to Cart
LT-137-3x1ml

LigaTrap® Mouse IgG Purification Column

3 x 1 ml $295.00 Add to Cart

IgG Yield and Purity

Purified polyclonal mouse IgG was spiked into a cell culture supernatant containing 5% fetal bovine serum and loaded over LigaTrap® Rat IgG Purification resin, Pierce™ Protein A and Protein G Plus at a load of 8mg IgG/ml resin with a five-minute residence time. Yields in histogram were determined using absorbance measurements at 280 nm. All manufacturer recommended chromatographic protocols were followed.

Mouse ascites fluid expressing monoclonal mouse IgG1-anti-IL6 (Interleukin 6) was loaded over LigaTrap® mouse IgG Purification resin, Pierce™ Protein A and Protein G Plus with a five-minute residence time. Yields in histogram were determined using ELISA. All manufacturer recommended chromatographic protocols were followed.

SDS PAGE - Ascites
SDS PAGE - Polyclonal


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