Mouse IgG Purification Resin and Columns
LigaTrap Technologies is excited to offer our popular Mouse IgG purification resin. LigaTrap Mouse IgG Purification line can bind all mouse IgG isotypes (IgG1, IgG2a, IgG2b, and IgG3) from polyclonal and monoclonal sources at a capacity of ≥20mg Mouse IgG/mL of resin. It is important to note that Protein A and Protein G have weaker affinity for mouse IgG1 compared to all other mouse IgG subclasses.
In addition to increased yields and purity levels, LigaTrap Technologies address many of the shortfalls related to standard affinity ligands including challenges. Our peptide ligands provide for a robust lifecycle, mild elution conditions and no immunogenicity or leaching.
| Part # | Name | Amount | Price | |
| LT-137KIT | Mouse IgG Mircospin Column Kit w/ Buffers |
KIT-10x0.1mL | $308.70 | Add to Cart |
| LT-137-1ml KIT | Mouse IgG 1mL Prepacked Column Kit w/ Buffers |
KIT-1x1mL | $236.25 | Add to Cart |
| LT-137-5ml KIT | Mouse IgG 5mL Prepacked Column Kit w/ Buffers |
KIT-1x5mL | $840.00 | Add to Cart |
| LT-137 | Mouse IgG Loose Resin-5mL |
5mL | $472.50 | Add to Cart |
| LT-137-10ml | Mouse IgG Loose Resin-10mL |
10mL | $750.00 | Add to Cart |
| LT-137-25ml | Mouse IgG Loose Resin-25mL |
25mL | $959.20 | Add to Cart |
| LT-137-50ml | Mouse IgG Loose Resin-50mL |
50mL | $1,398.20 | Add to Cart |
| LT-137-100ml | Mouse IgG Loose Resin-100mL |
100mL | $1,756.00 | Add to Cart |
| LT-137-MSC | Mouse IgG Microspin Columns without buffers |
10x0.1mL | $131.25 | Add to Cart |
| LT-137-1x1ml | Mouse IgG Prepacked Purification Column-1mL |
1x1mL | $105.00 | Add to Cart |
| LT-137-1x5ml | Mouse IgG Prepacked Purification Column-5mL |
1x5mL | $498.75 | Add to Cart |
| LT-137-3x1ml | Mouse IgG Prepacked Purification Columns-3x1mL |
3x1mL | $309.75 | Add to Cart |
Mouse IgG Yield and Purity
Purified polyclonal Mouse IgG was spiked into a cell culture supernatant containing 5% fetal bovine serum and loaded over Mouse IgG Purification resin, Pierce™ Protein A and Protein G Plus at a load of 8mg IgG/ml resin with a five-minute residence time. Yields in histogram were determined using absorbance measurements at 280 nm. All manufacturer recommended chromatographic protocols were followed.
Mouse ascites fluid expressing monoclonal mouse IgG1-anti-IL6 (Interleukin 6) was loaded over LigaTrap mouse IgG Purification resin, Pierce™ Protein A and Protein G Plus with a five-minute residence time. Yields in histogram were determined using ELISA. All manufacturer recommended chromatographic protocols were followed.
