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Rat IgG Purification Resin and Columns

LigaTrap Technologies is excited to offer our top of the line Rat IgG purification resin. Our Rat IgG purification line can bind all Rat IgG isotypes (IgG1, IgG2a, IgG2b, and IgG2c) from polyclonal and monoclonal sources at a capacity of ≥ 25mg Rat IgG/mL of resin. While Protein A and especially Protein G are commonly used for Rat IgG purification, it is worth noting that Protein A has weak affinity for all but Rat IgG2c, while Protein G has low to no binding of IgG1 and IgG2b.

In addition to increased yields and purity levels, LigaTrap Technologies address many of the shortfalls related to standard affinity ligands including challenges.  Our peptide ligands provide for a robust lifecycle, mild elution conditions and no immunogenicity or leaching.

Part # Name Amount Price
LT-138KIT

Rat IgG Mircospin Column Kit w/ Buffers

KIT-10x0.1mL $308.70 Add to Cart
LT-138-1ml KIT

Rat IgG 1mL Prepacked Column Kit w/ Buffers

KIT-1x1mL $236.25 Add to Cart
LT-138-5ml KIT

Rat IgG 5mL Prepacked Column Kit w/ Buffers

KIT-1x5mL $840.00 Add to Cart
LT-138

Rat IgG Loose Resin-5mL

5mL $472.50 Add to Cart
LT-138-10ml

Rat IgG Loose Resin-10mL

10mL $750.00 Add to Cart
LT-138-25ml

Rat IgG Loose Resin-25mL

25mL $959.20 Add to Cart
LT-138-50ml

Rat IgG Loose Resin-50mL

50mL $1,398.20 Add to Cart
LT-138-100ml

Rat IgG Loose Resin-100mL

100mL $1,756.00 Add to Cart
LT-138-MSC

Rat IgG Microspin Columns without buffers

10x0.1mL $131.25 Add to Cart
LT-138-1x1ml

Rat IgG Prepacked Purification Column-1mL

1x1mL $105.00 Add to Cart
LT-138-1x5ml

Rat IgG Prepacked Purification Column-5mL

1x5mL $498.75 Add to Cart
LT-138-3x1ml

Rat IgG Prepacked Purification Columns-3x1mL

3x1mL $309.75 Add to Cart

Rat IgG Yield and Purity

Purified polyclonal Rat IgG was spiked into a cell culture supernatant containing 5% fetal bovine serum and loaded over LigaTrap Rat IgG Purification resin, Pierce Protein A and Protein G Plus at a load of 8mg IgG/ml resin with a five-minute residence time. Yields in histogram were determined using absorbance measurements at 280 nm. All manufacturer recommended chromatographic protocols were followed.

COMPARABILITY
SDS PAGE


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