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Rat IgM Purification Resin and Columns

IgM monoclonal antibodies have become increasingly favorable for their use as therapeutic tools, while remaining popular for their research and diagnostic applications. More IgM based therapeutics are being developed now than ever before due to a better understanding of the roles it plays in the immune system. With a molecular weight of 990,000 Daltons, IgM is the largest and hardest of the major antibodies commonly purified.

LigaTrap technology separates itself by having the highest quality chromatographic purification for IgM on the market today. The advantages LigaTrap IgM Resin include capture both heavy and light chain subtypes of IgM, no immunogenicity or leaching, mild elution conditions, and can be reused after sanitization up to 100 times. LigaTrap IgM Purification Resin is capable of processing and purifying monoclonal antibodies form cell culture supernatant, ascites fluid, hybridoma, and other sources of recombinant IgM.

Product Descriptions

Purification kits are offered in microspin column, 1mL prepacked column, and 5mL prepacked column formats. Each kit includes the appropriate buffers and protocol for performing purifications.

Prepacked columns are offered in 1mL and 5mL formats for our various lines of antibody affinity chromatography resins. Prepacked columns come in kit formats or as standalone products.

Loose resin is available in various volume sizes which can be packed for end user’s specific purification needs. For bulk resin orders larger than 100mL, please inquire.

This product is not recommended in serum purification applications due to cross-reactivity with other immunoglobulins. Please consult LigaTrap for protocols that may improve results in serum.

Part # Name Amount Price
LT-147KIT

Rat IgM Kit Mircospin Column Kit w/ Buffers

KIT-10x0.1 mL $410.00 Add to Cart
LT-147-1ml KIT

Rat IgM 1mL Prepacked Column Kit w/ Buffers

KIT-1x1 mL $363.00 Add to Cart
LT-147-5ml KIT

Rat IgM 5mL Prepacked Column Kit w/ Buffers

KIT-1x5 mL $1,082.00 Add to Cart
LT-147

Rat IgM Loose Resin-5 mL

5 mL $724.00 Add to Cart
LT-147-10ml

Rat IgM Loose Resin-10mL

10 mL $948.00 Add to Cart
LT-147-25ml

Rat IgM Loose Resin-25mL

25 mL $1,496.00 Add to Cart
LT-147-50ml

Rat IgM Loose Resin-50mL

50 mL $2,492.60 Add to Cart
LT-147-100ml

Rat IgM Loose Resin-100mL

100 mL $3,738.90 Add to Cart
LT-147-MSC

Rat IgM Microspin Columns without buffers

10x0.1 mL $152.00 Add to Cart
LT-147-1x1ml

Rat IgM Prepacked Purification Column-1mL

1x1 mL $162.00 Add to Cart
LT-147-1x5ml

Rat IgM Prepacked Purification Column-5mL

1x5 mL $756.00 Add to Cart
LT-147-3x1ml

Rat IgM Prepacked Purification Columns-3x1mL

3x1 mL $464.00 Add to Cart

Rat IgM Yield and Purity

LigaTrap Rat IgM Purification resin is recovering Rat IgM at >2X of POROS™ CaptureSelect, >3X of GE IgM Purification HP and >30X that of Thermo Immobilized MBP resins. From SDS-PAGE data we can see that LigaTrap Rat IgM Purification resin is also binding rat IgM at a greater rate as seen by fainter heavy and light chain bands in the flowthrough fraction. SDS-PAGE also confirms SEC-HPLC data, showing that recovery of LigaTrap significantly greater than that of any other commercially available IgM purification resin.

Along with great yields and purity, the LigaTrap Rat IgM Purification resin uses the same simple and easy-to-follow protocol as all other LigaTrap products, the ability to recover IgM at a milder pH than competitors, and superior resin stability.

COMPARABILITY
SDS PAGE


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